![]() The 21 kb 'genetic load' region of pOLA 52 was shown to consist of a mosaic, among other things a fragmented Tn3 transposon encoding ampicillin resistance. prevalence of IncX1 group plasmids than IncX2. About half of the plasmid constituted a conserved IncX1-type backbone with predicted regions for conjugation, replication and partitioning, as well as a toxin/antitoxin (TA) plasmid addiction system. The plasmid was found to be 51,602 bp long with 68 putative genes. ![]() Sequence homology searches in GenBank also suggested a considerably higher. The conjugal transfer and plasmid maintenance regions of pOLA 52 therefore seem to represent IncX1 orthologues of the well-characterized IncX2 plasmid R6K. The plasmid was also classified as IncX1 with incompatibility testing. Nucleotide sequence of pOLA 52: a conjugative IncX1 plasmid from Escherichia coli which enables biofilm formation and multidrug efflux pOLA 52 is believed to represent the first example of a conjugative plasmid encoding type 3 fimbriae. and Enterobacter aerogenes, pOLA 52 facilitated increased biofilm formation. ![]() Pathogenic, members of the family Enterobacteriaceae, including Klebsiella pneumoniae, Salmonella Typhimurium, Kluyvera sp. Type 3 fimbriae, encoded by the conjugative plasmid pOLA 52, enhance biofilm formation and transfer frequencies in Enterobacteriaceae strainsīurmølle, Mette Bahl, Martin Iain Jensen, Lars Bogø
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